Course Title: Certification in Molecular Biology & PCR Testing

Designed by: Skillversity
Duration: 1 Month (4 Weeks)
Mode: Online / Hybrid
Level: Beginner to Intermediate

Weekly Modules Breakdown

Week 1: Fundamentals of Molecular Biology

• Introduction to Molecular Biology & Its Role in Diagnostics

• Structure & Function of DNA, RNA & Proteins

• Genetic Code, Transcription & Translation Mechanisms

• Nucleic Acid Extraction Techniques (DNA & RNA)

• Laboratory Safety & Biosafety in Molecular Testing

Week 2: Polymerase Chain Reaction (PCR) & Its Applications

• Basics of PCR: Principles & Mechanism of DNA Amplification

• Types of PCR: Conventional PCR, RT-PCR, qPCR, Multiplex PCR

• PCR Optimization: Primer Design, Annealing Temperature & Mg²+ Concentration

• Gel Electrophoresis for DNA/RNA Analysis

• Troubleshooting Common PCR Errors

Week 3: Molecular Diagnostics & Genetic Testing

• Applications of PCR in Infectious Disease Diagnosis (COVID-19, TB, HIV)

• Genetic Mutations & PCR-Based Testing for Genetic Disorders

• Oncogenes & Cancer Molecular Diagnostics (BRCA1/2, EGFR, etc.)

• CRISPR & Gene Editing Technologies

• Interpretation of PCR Results & Case Studies

Week 4: Advanced Molecular Biology Techniques

• Next-Generation Sequencing (NGS) & Its Clinical Applications

• Microarrays & DNA Hybridization Techniques

• Molecular Biomarkers for Personalized Medicine

• Bioinformatics Tools for Molecular Data Analysis

• Case Studies: Real-Life Applications of Molecular Biology in Healthcare

Assessment & Certification

• Weekly Quizzes & Assignments

• Practical Demonstrations (if applicable)

• Final Online Examination

• Certificate of Completion by Skillversity